ABS 036-01-02

antibody from Invitrogen Antibodies

Targeting: FCN1 FCNM

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

ABS 036-01-02

Provider

Invitrogen Antibodies

Product name

FCN1 Monoclonal Antibody (1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

ABS 036-01-02 detects M-Ficolin from human samples. ABS 036-01-02 has been successfully used in ELISA and IHC procedures. The ABS 036-01-02 immunogen is purified recombinant protein corresponding to residues 95-314 of human M-Ficolin, expressed in prokaryote. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1

Vial size

400 µL

Concentration

1.08 mg/mL

Storage

4° C, store in dark

References

Submitted references

Non-synonymous polymorphisms in the FCN1 gene determine ligand-binding ability and serum levels of M-ficolin.

Ammitzbøll CG, Kjær TR, Steffensen R, Stengaard-Pedersen K, Nielsen HJ, Thiel S, Bøgsted M, Jensenius JC
PloS one 2012;7(11):e50585

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Investigations on the pattern recognition molecule M-ficolin: quantitative aspects of bacterial binding and leukocyte association.

Kjaer TR, Hansen AG, Sørensen UB, Nielsen O, Thiel S, Jensenius JC
Journal of leukocyte biology 2011 Sep;90(3):425-37

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of M-Ficolin in lung tissue using a M-Ficolin monoclonal antibody (Product # ABS 036-01-02).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of M-Ficolin in liver tissue using a M-Ficolin monoclonal antibody (Product # ABS 036-01-02).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Characterization of five recombinant M-ficolin proteins. A The M-ficolin concentration measured in the supernatant of HEK293F cells transfected with plasmid encoding variants of M-ficolin. The wild type used as reference and the dotted line represents this value (100%). Boxes indicate range of data including median value. B Western blotting of supernatant from the wild type and the five variants of M-ficolin. For Ser268Pro also a lysate of the cells were used. The mutation for each variant is given beneath the lane. C Binding of recombinant M-ficolin to Streptococcus agalactiae serotype VI (GBS) The counts on the y-axis were obtained following incubation in GBS coated wells with recombinant M-ficolin and anti-M-ficolin antibody. Results displayed in A are from three while B and C are from two replicated experiments.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

8 Immunohistochemical staining for M-ficolin. Tissue and cellular distribution of M-ficolin examined with anti-M-ficolin mAb 036-05. (A) Liver (original magnification, x400). Granulocytes and monocytes in blood vessels are stained. Kupffer cells and hepatocytes are not stained. (B) Liver stained with control primary antibody (x400). (C) Lung (original magnification, x400). Strong staining of monocytes/granulocytes in the alveolar capillaries and no staining of type II pneumocytes (arrow) can be seen. Alveolar macrophages showed weak but nonspecific staining (not shown). (D) Lung (original magnification, x400). With the use of a milder retrieval approach, resulting in less cell staining (HIER in T-EG buffer), it is clearly seen that the stained cell is a granulocyte in a vessel (arrow). As in C, no staining of pneumocytes was observed. (E) Spleen (original magnification, x100). Many cells in red pulp are stained, apparently blood cells rather than resident cells. Only very few cells in follicles are stained. (F) Spleen, as in E, (original magnification, x400).